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Although ALCAR exerts antioxidant activities in rodents, it is not known whether taking high doses of ALCAR will have similar effects in humans

Specific symptoms that should prompt medical consultation include an irresistible urge to move the legs, especially when accompanied by uncomfortable sensations such as crawling, pulling, tingling, or aching deep within the leg muscles

Despite testing with different commercial antibodies, our western blot results were inconsistent, and we could not obtain a reliable pattern of expression (see Supplementary Figure 1 )

Proteins were separated by SDS-PAGE, transferred to polyvinylidene fluoride (PVDF) membranes, and blocked with 5% skim milk in TBST for 1 h